Bacterial and Virus Strains |
E.coli Rosetta (DE3)pLysS (expression strain) |
Novagen |
N/A |
Deposited Data |
LP1413 coordinates and structure factors |
This paper |
PDB: 6BTC |
Raw diffraction images |
This paper |
SBGrid data set#531 doi:10.15785/SBGRID/531 |
Raw gel images |
This paper |
https://data.mendeley.com/datasets/vh896md947/draft?a=feba66cd-983d-4855-8e91-14e414a5e14e |
Experimental Model |
|
|
LP1413 protein (identical to the sequence used here) is found on SCC elements in many staphylococci. The NCBI Reference Sequence for this protein is WP_001036438.1. |
N/A |
https://www.ncbi.nlm.nih.gov/protein/WP_001036438.1 |
Oligonucleotides |
See table S2 for sequences of oligonucleotides used as primers and as EMSA substrates |
This paper |
N/A |
Recombinant DNA |
pET-21a expression vector containing LP1413 gene |
Novagen; Mir-Sanchis et al., 2016
|
pET21aLP1413 |
Software and Algorithms |
CCP4 |
Winn et al., 2011 |
www.ccp4.ac.uk |
HKL-2000 |
Otwinowski, Z and Minor, W., 1997 |
http://www.hkl-xray.com/ |
Phenix |
Adams et al., 2010 |
https://www.phenix-online.org/ |
Coot |
Emsley et al., 2010 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
GraphPad Prism Software version 7 |
|
www.graphpad.com |
Pymol |
Baker et al., 2001 |
https://pymol.org/2/ |
GraphiteLifeExplorer |
Hornus et al., 2013 |
http://www.lifeexplorer.info/ |
SBgrid |
Morin et al., 2013 |
https://sbgrid.org/ |