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. Author manuscript; available in PMC: 2019 Aug 7.
Published in final edited form as: Structure. 2018 Jul 12;26(8):1144–1150.e3. doi: 10.1016/j.str.2018.05.016
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and Virus Strains
E.coli Rosetta (DE3)pLysS (expression strain) Novagen N/A
Deposited Data
LP1413 coordinates and structure factors This paper PDB: 6BTC
Raw diffraction images This paper SBGrid data set#531 doi:10.15785/SBGRID/531
Raw gel images This paper https://data.mendeley.com/datasets/vh896md947/draft?a=feba66cd-983d-4855-8e91-14e414a5e14e
Experimental Model
LP1413 protein (identical to the sequence used here) is found on SCC elements in many staphylococci. The NCBI Reference Sequence for this protein is WP_001036438.1. N/A https://www.ncbi.nlm.nih.gov/protein/WP_001036438.1
Oligonucleotides
See table S2 for sequences of oligonucleotides used as primers and as EMSA substrates This paper N/A
Recombinant DNA
pET-21a expression vector containing LP1413 gene Novagen; Mir-Sanchis et al., 2016 pET21aLP1413
Software and Algorithms
CCP4 Winn et al., 2011 www.ccp4.ac.uk
HKL-2000 Otwinowski, Z and Minor, W., 1997 http://www.hkl-xray.com/
Phenix Adams et al., 2010 https://www.phenix-online.org/
Coot Emsley et al., 2010 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
GraphPad Prism Software version 7 www.graphpad.com
Pymol Baker et al., 2001 https://pymol.org/2/
GraphiteLifeExplorer Hornus et al., 2013 http://www.lifeexplorer.info/
SBgrid Morin et al., 2013 https://sbgrid.org/