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. 2018 Jun 8;177(4):1650–1665. doi: 10.1104/pp.18.00401

Figure 4.

Figure 4.

Stress responsiveness of 6xABRE SP reporters. A, Confocal images of 6xABRE_A SP homozygous reporter expression in root tips under three different concentrations of NaCl for short (5 h) and long (24 h) treatment. B and C, Bar charts showing the averaged fluorescence level of 6xABRE_A SP reporter in various cell types under 5 h NaCl (B) or 24 h NaCl (C) treatment, quantified by CellSeT (n ≥ 5). D, Confocal images of 6xABRE_A SP homozygous reporter expression in root tips after treatment with three different concentrations of mannitol for short (5 h) and long (24 h) treatment. E and F, Bar charts showing the averaged fluorescence level of 6xABRE_A SP reporter in various cell types after 5 h mannitol (E) or 24 h mannitol (F) treatment, quantified by CellSeT (n ≥ 5). The same sets of confocal images and bar chart for 6xABRE_R SP reporter are shown in Supplemental Fig. S6, D to I. G and H, PCA with loadings of the expression data from both versions of 6xABRE SP reporters after NaCl or mannitol 5 h (G) or 24 h (H) treatments. I, Confocal images of 6xABRE_A SP reporter expression in lateral roots (LR) after 3 d 100 mm NaCl and 200 mm mannitol treatments. Scale bars, 50 μm. Student’s t test significance level, *0.01 < P < 0.05, **0.001 < P < 0.01, ***P < 0.001. Error bars, se.