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. 2018 Jun 13;177(4):1666–1678. doi: 10.1104/pp.18.00377

Figure 5.

Figure 5.

PAT10 and CBL2/CBL3 positively regulate K+ influx at the tonoplast through NHX. A, Stomatal aperture upon treatment of FC at different concentrations (0–1.5 µm). Black line, wild type; red line, pat10-1; blue line, cbl2;cbl3. FC was added together with 10 µm ABA to fully opened stomata. Aperture was measured after 2 h treatment. B, Vacuolar convolution as indicated by the percentage of visually separate vacuoles at different size range in 30 guard cells. FC at 0.75 µm was applied together with 10 µm ABA. C, ABA-induced stomatal closure in the wild type, pat10-1, tpk1-1, pat10-1;tpk1-1, NHX2OX1, NHX2OX1;pat10-1, NHX2OX2, and NHX2OX2;pat10-1. Rosette leaves with preopened stomata were treated with 0 and 10 µm ABA for 2 h before the apertures were measured. Results are means ± se of four replicates (20 stomata from two epidermal peels per replicate). Different letters indicate significantly different response to ABA-induced stomatal closure (one-way ANOVA, Tukey-Kramer test, P < 0.05).