Table 1. Complementation of a sim mutant by transgenic constructs with mutations in various motifs of SIM.
The multicellular trichome phenotype of a complementation line homozygous for a single T-DNA insert for each of the indicated SMRs was assessed by counting the number of 4,6-diamidino-2-phenylindole (DAPI)-stained nuclei at each trichome initiation site (TIS) for each genotype. All values followed by the letter a have significantly fewer nuclei per TIS than the sim mutant (P < 1 × 10−11 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). For each transgenic genotype, at least two additional independent homozygous T3 lines were obtained having a phenotype qualitatively equivalent to the line shown here.
| Genotype | No. of Nuclei per TIS | No. of TIS |
|---|---|---|
| Col-0 | 1.00 ± 0.00 a | 50 |
| Sim | 2.16 ± 0.98 | 50 |
| sim GL2pro:YFP:SIM | 1.03 ± 0.18 a | 50 |
| sim GL2pro:YFP:motifA | 1.98 ± 0.80 | 50 |
| sim GL2pro:YFP:motifB | 1.96 ± 0.67 | 50 |
| sim GL2pro:YFP:motifC | 1.02 ± 0.14 a | 50 |