Table 3. Redundant NLSs in the C-terminal end of SIM are required for its full function.

The multicellular trichome phenotype of a complementation line homozygous for a single T-DNA insert for each of the indicated SMRs was assessed by counting the number of DAPI-stained nuclei at each trichome initiation site (TIS) for each genotype. All genotypes followed by the letter a have significantly fewer nuclei per TIS than the sim mutant (P < 1 × 10⁻⁹ in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). The genotype followed by the letter b has significantly fewer nuclei than the sim mutant (P < 0.01 in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests) but significantly more nuclei per TIS than Col-0 (P < 1 × 10⁻⁶ in a one-tailed Student’s t test, after applying a Bonferroni correction for multiple tests). For each transgenic genotype, at least two additional independent homozygous T3 lines were obtained having a phenotype qualitatively equivalent to the line shown here.

Genotype	No. of Nuclei per TIS	No. of TIS
Col-0	1.00 ± 0.00 a	50
sim	2.16 ± 0.98	50
sim GL2pro:Δ104-127	1.08 ± 0.27 a	50
sim GL2pro:Δ82-127	1.02 ± 0.14 a	50
sim GL2pro:Δ66-127	1.84 ± 0.71	50
sim GL2pro:nls1	1.04 ± 0.20 a	50
sim GL2pro:nls2	1.02 ± 0.14 a	50
sim GL2pro:nls1 nls2	1.50 ± 0.74 b	50