Figure 5.
SDH1 accumulates as a soluble FAD-bound protein in sdhaf4. A, Peptide abundance in soluble (S) and membrane (M) mitochondrial protein fractions compared between mutant lines and the wild type (n = 3). B, Ratio of protein abundance in soluble fractions to that in membrane fractions within each genotype (n = 3). C, Comparison of the gel band area of FAD-bound SDH1 between mutant lines and the wild type from soluble and mitochondrial membrane fractions (n = 4). *, P ≤ 0.08 and **, P ≤ 0.05, by Student’s t test. Error bars indicate se.