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. 2018 Jun 21;177(4):1439–1452. doi: 10.1104/pp.18.00320

Figure 6.

Figure 6.

35S-SDHAF4 complements SDH activity in sdhaf4. A, Fold change of succinate abundance in sdhaf4 and two independent complementation lines (22.3 and 23.4) compared with Ler (n = 4). B, Fold change of succinate abundance in sdhaf2 compared with Col-0 (n = 4). C, SDH enzyme activity at 10 mm succinate. D, Oxygen consumption in the presence of 5 mm succinate. E, Protein peptide detection of SDH subunits SDH1 and SDH2 and SDH assembly factors SDHAF2 and SDHAF4 (n = 4). Error bars indicate se; N.D., not detected. Student’s t test was used to determine significant differences between sdhaf4 and Ler complementation lines: *, P < 0.05; **, P < 0.01; and ***, P < 0.001.