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In vitro conversion of jasmone to jasmolone in microsomes prepared from N. benthamiana leaves transiently expressing TcJMH. After completion of the assay, the solution was extracted with MTBE and the extract was analyzed by GC-MS. For a control, the jasmone hydroxylation assay was conducted with microsomes prepared from N. benthamiana leaves transiently expressing GFP. For details of the enzymatic assay and GC-MS analysis, see “Materials and Methods.”
