Figure 2.

Mapping of the SKI QTL for freezing tolerance of B. stricta seedlings. A, QTL analysis results of freezing tolerance across the seven LGs using the SAD12 × LTM RIL population. The red line shows the LOD significance threshold of 2.8. B, A single significant QTL above the LOD threshold for seedling survival assay was identified on LG5 (top) and its estimated additive effect (bottom). The negative value of the additive effect indicates that the LTM ecotype allele confers enhanced freezing tolerance. C, The precise two-LOD interval and marker names of the SKI major QTL on B. stricta LG5.