Table 2. Cortical MT dynamics.
MT parameters were measured from time-lapse images of 3-d-old mCherry-TUB6, Col-0 and mCherry-TUB6, iqd5-1 cotyledons. Values for growth rates, shrinkage rates, and dynamicity are means ± sd. For catastrophe and rescue frequencies, the values with SD were calculated by averaging the inverse time needed for each individual switch and the values without SD were obtained from the inverse of the mean time spent in growth and shrinkage, respectively, as described previously (Dhonukshe and Gadella, 2003; Abe and Hashimoto, 2005). Asterisks indicate statistically significant differences (for each measurement listed, we used 10–20 seedlings per genotype to observe 20–50 cells from which we assessed n ≥ 50 MT dynamic properties: Student’s t test, two tailed, type 3; *, P < 0.05 and **, P < 0.01).
| Dynamic Parameters | mCherry-TUB6, Col-0 | mCherry-TUB6, iqd5-1 |
|---|---|---|
| Growth rate (µm min−1) | 5.39 ± 1.13 | 4.34 ± 0.85** |
| Shrinkage rate (µm min−1) | 12.87 ± 2.69 | 14.40 ± 2.92* |
| Dynamicity (µm min−1) | 6.12 ± 1.25 | 6.92 ± 1.71* |
| Catastrophe frequency (events s−1) | 0.0192 | 0.0198 |
| 0.020 ± 0.005 | 0.022 ± 0.008 | |
| Rescue frequency (events s−1) | 0.065 | 0.067 |
| 0.071 ± 0.021 | 0.073 ± 0.020 |