PC and PE molecular species in the wild type (WT) and mutants lacking NMT1 or NMT3 function, or both. Molecular species of PC (A) and PE (B) are shown in roots and shoots of 12-d-old wild-type, nmt1, nmt3, and nmt1 nmt3 plants, graphed as facet plots with the total carbons as vertical facets and the number of double bonds as horizontal facets. Bars show means ± se (n = 4–6 biological replicates, each corresponding to a pool of 36–100 seedlings, separating shoots and roots). Bars topped by different letters denote statistically significant differences among genotypes by one-way ANOVA run for each tissue, followed by Bonferroni’s pairwise test (P < 0.05). DW, Dry weight.