Skip to main content
. 2018 May 18;177(4):1605–1628. doi: 10.1104/pp.18.00457

Figure 4.

Figure 4.

NMT3 rescues the short-root phenotype induced by the loss of NMT1 function. A and B, Representative photographs (A) and primary root lengths (B) of 8-d-old seedlings. From left to right: nmt1 mutant, wild type (WT), and four independent transgenic complementation lines expressing ProNMT1:NMT1 (lines 23 and 13) or ProNMT1:NMT3 (lines 134 and 112) in the nmt1 background. Seedlings were grown along vertical plates on 1.2% agar supplemented with one-half-strength Murashige and Skoog medium and 1% Suc. Values in B are means ± se (n = 14–16). Bars topped by different letters denote statistically significant differences in one-way ANOVA (P < 0.001). Bars in A = 1 cm. C, Confocal images of propidium iodide-stained roots of nmt1 and nmt1 expressing ProNMT1:gNMT1 (line 23) or ProNMT1:gNMT3 (line 112). Both transgenes suppress the epidermal defects and extensive cell death caused by NMT1 loss of function (bright red areas). Bar = 100 µm.