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. 2018 May 18;177(4):1605–1628. doi: 10.1104/pp.18.00457

Figure 5.

Figure 5.

NMT3 loss of function has, alone, no apparent phenotypic impact but severely impairs vegetative growth and root development in an nmt1 background. A, Overall phenotypes of 12-d-old wild-type (WT), nmt1 (1), nmt3 (3), and nmt1 nmt3 (1.3) seedlings. Bar = 1 cm. B, Kinetics of wild-type, nmt1, nmt3, and nmt1 nmt3 primary root elongation. Values are means ± se (n = 12). C and D, Shoot (C) and root (D) fresh weights of 2-week-old wild-type, nmt1, nmt3, and nmt1 nmt3 seedlings. Values are means ± se (n = 6). E, Confocal optical sections of 10-d-old wild-type, nmt1, nmt3, and nmt1 nmt3 primary roots stained with propidium iodide. Blue arrowheads indicate the boundary between the root meristem and the elongation zone. Bars = 100 µm. F, Lateral root density for 10-d-old wild-type, nmt1, nmt3, and nmt1 nmt3 roots. Values are means ± se (n = 11–12). G, Meristematic cortical cell number in wild-type, nmt1, nmt3, and nmt1 nmt3. Values are means ± se (n = 8). H, Mature cortical cell length in wild-type, nmt1, nmt3, and nmt1 nmt3 roots. Values are means ± se (n = 56–129). I, Example of a lateral root primordia cluster on a 10-d-old nmt1 nmt3 primary root, imaged by differential interference contrast (DIC) light microscopy. Bar = 50 µm. J, Confocal images of epidermal cells in 10-d-old wild-type, nmt1, nmt3, and nmt1 nmt3 primary roots stained with propidium iodide. Bars = 50 µm. K and L, Propidium iodide-stained wild-type, nmt1, nmt3, and nmt1 nmt3 roots grown in the absence (K) or presence (L) of 200 µm choline. Bars = 100 µm. M, Photographs of representative wild-type, nmt1, nmt3, and nmt1 nmt3 seedlings grown on medium supplemented with 200 µm choline. Bar = 1 cm. Bars topped with different letters within each graph (B–D and F–H) denote statistically significant genetic differences by one-way ANOVA (P < 0.05).