Table I. Segregation of the nmt1 and NMT1 or nmt3 and NMT3 alleles based on antibiotic resistance in the F1 or F2 progeny of crossed or self-pollinated flowers.
P, χ2 test probability values. P > 0.05 indicates no significant difference from a 1:1 segregation ratio (F1 generation) or a 3:1 ratio (F2 generation).
| Cross ♀ ♂ | Generation | nmt1 KanRa | NMT1 KanSa | nmt3 SulRa | NMT3 SulSa | Segregation Ratio | P |
|---|---|---|---|---|---|---|---|
| Wild type nmt3 nmt1+/− | F1 | 154 | 173 | 1:1 | 0.29 | ||
| Wild type nmt1 nmt3+/− | F1 | 206 | 212 | 1:1 | 0.77 | ||
| nmt1+/− nmt1+/− | F2 | 573 | 208 | 3:1 | 0.29 | ||
| nmt3+/− nmt3+/− | F2 | 223 | 67 | 3:1 | 0.46 | ||
| nmt3 nmt1+/− wild type | F1 | 252 | 271 | 1:1 | 0.41 | ||
| nmt1 nmt3+/− wild type | F1 | 246 | 229 | 1:1 | 0.44 |
a
Numbers of F1 or F2 seedlings resistant or sensitive to kanamycin (KanR and KanS, for the nmt1 and NMT1 alleles, respectively) or to sulfonamide (SulR and SulS, for the nmt3 and NMT3 alleles, respectively).