Figure 8.

Some loci exhibit conserved imprinting in plants. A, Venn diagram analysis of imprinting conservation in plants. Only a few of the most similar rice homologs (top one hit) of the imprinted genes found in maize, sorghum, and Arabidopsis show imprinting in rice. B, Many of the conserved imprinting loci are imprinted in barley. Conserved imprinted genes refer to those identified from at least two species of rice, maize, sorghum, and Arabidopsis. The top three BLAST hits (e < 10e-10) are used to evaluate imprinting conservation. C, Validation of the imprinting status of 11 conserved imprinted loci in barley. M and B indicate Morex and Bowman, respectively, and the superscripts indicate the polymorphic nucleotides of each allele. Polymorphic sites are highlighted in gray.