Change in relative root elongation after treatment with 30% PEG. Seedlings were treated with PEG and then plated on Murashige and Skoog plates with 1% Suc. Growth of the main root or adventitious root was measured daily after PEG treatment and quantitated with Fiji software, where no treatment was defined as 1. A, Quantitation of the main root growth rate relative to untreated control, following various PEG treatments. B, Quantitation of the lateral root length relative to untreated control, following various PEG treatments. Error bars are se and n ≥ 9 seedlings. Asterisks indicate significant difference from the untreated sample (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001).