Singlet oxygen- and hydrogen peroxide-specific gene expression in roots. A, Transcript levels were measured by RT-qPCR analysis at 0, 1, 2, and 3 h after treatment with 30% PEG. B, Specificity of gene expression. The transcript level fold change in roots; no treatment (Control); treatment for 2 h in a 100 µm rose bengal aqueous solution under 30 µE light (RB 2 h); treatment for 2 h in a 5 mm H2O2 aqueous solution (H2O2 2 h). Error bars are se, n = 3. Asterisks indicate significant difference from the untreated sample (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001).