MST Acquisition. MST acquisition was significantly better when sleep preceded the MST training session (Panels A, D). Overall, biological time did not significantly influence task acquisition (Panels B, E).The post-sleep/wake difference in acquisition (A, D) was due to the superior performance after participants slept during the day. The asterisk in Panels C and F describes the difference between the Day Sleep and other three conditions (all p-values < .05, except the After Night Sleep–After Day Sleep comparison, p = .057 [Unadjusted]). Bars show the estimated means ± SEMs from the mixed model analyses.