Schematic of the cuvette FCS setup. Laser (543 nm, CW), L1, L2: lenses, L1 is aspheric lens; DCM, dichroic mirror; AD, achromatic doublet lens; EF, emission filter; BS, 50/50 beam splitter; FC, fiber coupler; TS, micrometer translation stage; Objective, ELWD, air objective, 0.7 NA, WD = 2.6–1.8 mm. To see this figure in color, go online.