(A,B) Representation colony formation images and colony formation counting are shown. (A: SW620, and B: SW480). (C,D) Transwell assay performed to determine the migration and invasion of cell lines SW620 and SW480(C: SW620, and D: SW480). (E,G) Flank tumor, liver and lung metastatic burden in nude mice (n = 6 for each group) injected with CRC cell lines with rs2333227 CC,CT and TT genotypes, measured by luciferase activity. ***P < 0.001, *P < 0.05, **P < 0.01.