Fig 2. qPCR analysis shows that piwi RNA is differentially regulated in S. mansoni infected in B. glabrata snails depending on their susceptibility phenotypes as follows: Panel A, resistant BS-90 stock; B, susceptible NMRI stock; and C, susceptible BB02 stock.

qPCR was performed as previously reported by Ittiprasert and colleagues (2009) with the following primers: 5′-GTCACACCTACCAGCTACAATG and 3′-GGTTCCCTGCCAGTTGAAATA. NMRI, Naval Medical Research Institute.