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Immunofluorescence staining showing that BMP7-IR positive cells in the cerebral cortex (A), hippocampus (B) and corpus callosum (C) are astrocytes based on nuclear staining using DAPI, and that BMP7-IR positive cells in the hippocampus (D1) are not positive for Iba1 (D2) in a merged photomicrograph (D3). cc, corpus callosum; Cor, cerebral cortex; DAPI, 4′, 6-diamidino-2-phenylindole; Hip, hippocampus. Scale bar = 20 μm for D; 8 μm for A-C.
