BMP7 expression in the hypothalamus (A–F). Large neurons in the magnocellular part of the paraventricular hypothalamic nucleus were very strongly stained (arrowheads in B), and the medial part of the median eminence was also very strongly stained (arrows in D). The mirror method showed that the positive neurons were oxytocin neurons (arrowheads in C1 and C2). PV, paraventricular hypothalamic nucleus; ME, medial eminence. Scale bar = 400 μm for D; 160 μm for A; 80 μm for E; 40 μm for B; 20 μm for C1, C2, F.