Table 1. Primer sequences used for reverse transcription-polymerase chain reaction.
| Gene | Full name | NM accession | Sequence |
|---|---|---|---|
| CD133 | Prominin 1 | NM 006017.2 | For 5’CTCAGACTGGTAAATCCCCC |
| Rev 5’GACGCTTTGGTATAGAGTGC | |||
| CD44 | CD44 molecule | NM 001202557.1 | For 5’GATCCTCCAGCTCCTTTCG |
| Rev 5’GGAATACACCTGCAAAGCG | |||
| CNP | 2’,3’-cyclic nucleotide 3’phosphodiesterase | NM 033133.4 | For 5’CCAAGTTTTGTGACTACGGG |
| Rev 5’AGCTTGTCCACATCACTCG | |||
| DCX | Doublecortin | NM 001195553.1 | For 5’GTACGTTTCTACCGCAATGG |
| NM 000555.3 | Rev 5’ATCCATGCTTCCGATCTTCC | ||
| NM 178153.2 | |||
| NM 178152.2 | |||
| NM 178151.2 | |||
| GALC | Galactosylceramidase | NM 000153.3 | For 5’AGCATCACTTCACGCTACG |
| Rev 5’CTTCCTGCAATGAACACACC | |||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM 001256799.1 | For 5’-CAAAAGGGTCATCATCTCTGC |
| NM 002046.4 | Rev 5’ AGTTGTCATGGATGACCTTGG | ||
| GFAP | Glial fibrillary acidic protein | NM 002055.4 | For 5’CTACAGGAAGCTGCTAGAGG |
| Rev 5’TTAATGACCTCTCCATCCCG | |||
| GLAST | Solute carrier family 1 | NM 001166696.2 | For 5’TGCAAAGAAGAGACCCTCC |
| (glial high affinity glutamate transporter) | Rev 5’CTGTGAGCAGCACAAAAGC | ||
| NCAM | Neural cell adhesion molecule 1 | NM 001242608.1 | For 5’TACAAAGCTGAGTGGAGAGC |
| NM 001242607.1 | Rev 5’CGTCTTGAACTCGGAGGC | ||
| NM 001076682.3 | |||
| NM 181351.4 | |||
| NM 000615.6 | |||
| NESTIN | Nestin | NM 006617.1 | For 5’AGAAACAGGGCCTACAGAG |
| Rev 5’AAAGCTGAGGGAAGTCTTG | |||
| SOX2 | SRY-box2 | NM 003106.3 | For 5’GCAGTACAACTCCATGACCA |
| Rev 5’GATCATGTCCCGGAGGTC | |||
| TUBBIII | Tubulin, beta 3 class III | NM 006086.3 | For 5’CCAGTATGAGGGAGATCG |
| Rev 5’CACGTACTTGTGAGAAGAGG |
Accession numbers correspond to the transcripts for the gene. When several transcripts for the same gene exist, the probes were designed to amplify all transcripts.