Fig. 4.

Cell type specific localization of Cre activity in the GLYT2-Cre knock-in mice. Combined immunohistochemistry for β-gal (A, D, G, J, M, P) and in situ hybridization for GLYT2 (B, E, H, K, N, Q) in the cerebellar cortex (A–C) and various brainstem nuclei, including the spinal trigeminal nucleus (D–F), gigantocellular reticular nucleus (G–I), dorsal cochlear nucleus (J–L), nucleus of the trapezoid body (M–O), and ventral nucleus of lateral lemniscus (P–R), of the GLYT2-Cre/R26R double transgenic mice. Arrows in A-C indicate the coexpression of the β-galactosidase protein and GLYT2 mRNA in Golgi cells. GC, granule cell layer; Mo, molecular layer; PC, Purkinje cell layer. Scale bars, 100 μm.