Fig. 3.

A) Western blot tau phosphorylation assay: tau phosphorylation at two epitopes of Ser²⁶² and Ser²⁰²/Thr²⁰⁵ (AT8) were analysed following 2 min ischaemia, 2 min ischaemia followed by 60 min recovery as well as 4 weeks recovery (n = 6 each). Total tau and actin levels are also examined. I-2m: 2 min ischaemia, I-2m/R- 60 m: 2 min ischaemia followed by 60 min reperfusion, I-2m/R- 4w: 2 min ischaemia followed by 4 weeks recovery period (One Way ANOVA, *p < 0.01, for p-tau (Ser²⁶²) in I-2m, I-2m/R- 60 m and I-2m/R- 4w vs control). Error bars depict the SD. All values are expressed as percent change relative to control group and were corrected by the Actin level. B) The presence of p-tau (Ser²⁶²), visualized by immunofluorescent staining which is mainly located in the processes in control group (a, b, c) and in soma and some parts of processes in the long-term recovery group of 2 min ischaemia followed by 4 weeks (d, e, f). DAPI (blue) shows nuclei staining. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)