Fig 1. Tissue distribution of losartan after delivery via drinking water.

Concentrations of losartan (left) and its active metabolite, EXP 3174 (right) in the plasma (red symbols) and eye (blue symbols) were determined by LC/MS after 3 days of treatment with 1.2 g/L losartan in water available ad libidum. At different time points, mice (n = 3) were sacrificed and eyes and plasma were collected for LC/MS. Approximate peak concentrations were determined by gathering samples at the end of the active nocturnal phase. Approximate trough concentrations were determined by gathering samples at the end of the inactive daytime phase. Symbols represent mean/SD.