Fig 2. SURFIN_4.2 protein expression during the parasite asexual cycle.

All panels depict PAGE followed by immunoblot with αSURFIN_4.2. (A) SDS-PAGE on a parasite time-course during the asexual cycle inside RBCs, pellet (P) and supernatant (S) after saponin pellet extraction with the detergent SB3-10. (B) SDS-PAGE on cellular pellet (lane 1) and culture supernatant (lanes 2 and 3, before and after ultracentrifugation respectively) upon schizont rupture. (C) BN-PAGE on a parasite time-course, only supernatants after extraction with SB3-10 were tested.