Fig 4. Determination of the activity of iron-sulfur cluster-containing enzymes in P. aeruginosa strains.
The enzyme activity of aconitase (A) and succinate dehydrogenase (B) was measured in the PAO1 and ΔnfuA mutant carrying the empty Tn7T vector (PAO1::Tn7T and ΔnfuA::Tn7T) as well as in the ΔnfuA mutant expressing wild-type NfuA or mutated NfuA (NfuAC152S, NfuAC155S, and NfuAC43,47S) under uninduced and Paraquat-induced conditions as described in the Materials and Methods. The data shown are the means ± SD from three independent experiments.