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Nitrate reductase activity was measured in the PAO1 and ΔnfuA mutant carrying Tn7T vector (PAO1::Tn7T and ΔnfuA::Tn7T), and the ΔnfuA mutant expressing wild-type NfuA or mutated NfuA (NfuAC152S, NfuAC155S, and NfuAC43,47S), as described in the Materials and Methods. The data shown are the means ± SD from three independent experiments.
