Table 1. Summary of read count after various filtering steps.

Filtering criteria	Number of reads	Percentage of reads
Length outside bounds of 150 and 1000	290	0.02
Ambiguous bases exceeds limit of 0	0	0.00
Missing quality score	0	0.00
Mean quality score below minimum of 20	0	0.00
Max homopolymer run exceeds limit of 6	21,363	1.31
Mismatches in primer exceeds limit of 0	56,136	3.45
Chimeric sequences	66,414	4.08
Total removed	144,203	8.85
Total retained	1,484,525	91.15