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rtg2Δ strains are USA+. (Left) The [ure-o] strains YHE861 and -867 were crossed to generate the [ure-o] diploid strain MP52. One copy of RTG2 was deleted by transformation with a PCR-amplified rtg2∷G418 cassette. (Right) The rtg2Δ/+ diploid was verified by PCR, sporulated, and the segregants were tested for growth on USA. All 24 rtg2 segregants were able to grow on USA, whereas all of the RTG2 segregants were USA−.
