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Ure2p is not aggregated in rtg2Δ strains. Strains (A) MP29 (RTG2 [URE3]), (B) MP71 (RTG2 [ure-o], a guanidine-cured derivative of MP29), and (C and D) MP46 (rtg2) carrying pVTG12, expressing the N-terminal prion-inducing domain of Ure2p fused to GFP were examined by fluorescence microscopy as described (17). The rtg2Δ strain was treated (C) or untreated (D) with 5 mM guanidine.
