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. 2018 Aug 9;13(8):e0201982. doi: 10.1371/journal.pone.0201982

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© 2018 Nakamura et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A, D, F) Dansylated (DNS) cis-substrates were incubated either without (lane a) or with (lane b) XeHypBA1, XeHypBA2, or XeHypAA and the reaction products were analyzed by TLC. Ara-Hyp-DNS (lane 1), Ara₂-Hyp-DNS (lane 2), Ara₃-Hyp-DNS (lane 3), and Ara₄-Hyp-DNS (lane 4) were used as substrates. (B) TLC analysis of XeHypBA1 reaction products. l -arabinose standard (lane 1). Ara-Hyp (lane 2) and Ara₂-Hyp (lane 3) were incubated either without (lane a) or with (lane b) XeHypBA1. (C, E) HPAEC-PAD analysis of XeHypBA1or XeHypBA2 reaction products. β-Ara₂ standard (a, d); l-arabinose standard (b); Ara₃-Hyp standard (e); β-Ara₂ incubated with XeHypBA1 (c); Ara₃-Hyp incubated with XeHypBA2 (e). HD, Hyp-DNS; A1HD, Ara-Hyp-DNS; A2HD, Ara₂-Hyp-DNS; A3HD, Ara₃-Hyp-DNS; A4HD, Ara₄-Hyp-DNS. A1, L-arabinose; A1H, Ara-Hyp; A2, β-Ara₂; A2H, Ara₂-Hyp; A3H, Ara₃-Hyp; c, cis-isomer; t, trans-isomer.