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. 2018 Aug 9;13(8):e0201982. doi: 10.1371/journal.pone.0201982

Fig 6. Expression analysis of xehypBA2-AA operon.

Fig 6

(A) RT-PCR was conducted to confirm whether the two genes, xehypBA2 and xehypAA, form an operon. A 346-bp fragment containing the junction region of the two genes was amplified using extracted mRNAs (lane 1), genomic DNAs (lane 2) and cDNAs (lane 3) derived from X. euvesicatoria UPB139 grown in XVM2 (hrp-inducing medium). M, DNA molecular weight marker. (B) Relative expression level of the operon in NYG (complete medium) and XVM2 (hrp-inducing medium) was examined by qRT-PCR. (C) Relative expression level of the operon in the wild type (WT) and a hrpX-deletion mutant (ΔhrpX) in NYG and XVM2. (D) Relative expression level of the operon in NYG and infected Micro-Tom. The expression values relative to the mean expression in NYG were calculated using the 2-ΔΔCt method. Error bars indicate standard deviation (±SD) of three independent experiments.