Table 1. Bacterial strains and plasmids used in study.
| Strains/plasmids | Relevant characteristics | Reference/source |
|---|---|---|
| Xanthomonas euvesicatoria | ||
| UPB139 | Wild type isolated from tomato | [22] |
| ΔhrpX | hrpX deletion mutant of UPB139 | This study |
| ΔxehypBA1 | xehypBA1 deletion mutant of UPB139 | This study |
| ΔxehypBA2 | xehypBA2 deletion mutant of UPB139 | This study |
| ΔxehypAA | xehypAA deletion mutant of UPB139 | This study |
| ΔxehypBA2-AA | xehypBA2 and xehypAA deletion mutant of UPB139 | This study |
| ΔxehypBA1-BA2-AA | xehypBA1, xehypBA2 and xehypAA deletion mutant of UPB139 | This study |
| Escherichia coli | ||
| XL1-Blue | hsdR17, supE44, recA1, endA1, gyrA46, thi, relA1, lac/F’ {proAB+, lac Iq, lacZΔM15:: Tn10(tetr)} | Agilent |
| JM109 | ecA1, endA1, gyrA96, thi, hsdR17(rK- mK+),e14- (mcrA-), supE44, relA1, Δ (lac-proAB)/F’{traD36, proAB+, lacIq, lacZΔM15} | Takara Bio |
| BL21 (λDE3) | F-, ompT, hsdSB(rB- mB-), gal(λcI 857, ind1, Sam7, nin5, lacUV5-T7gene1), dcm(DE3) | Merck |
| S17-1 (λpir) | hsdR, recA, pro, RP4-2 (Tc::Mu; Km::Tn7) (λ pir) | Biomedal |
| Brevibacillus choshinensis | ||
| SP3 | imp, em-, spoIIAC | Takara Bio |
| Plasmids | ||
| pET-23b (+) | Expression vector, Apr, His-tag | Merck |
| pBIC3 | Expression vector, P22 promoter, P22 signal peptide, Nmr, His-tag | Takara Bio |
| pCold TF | Expression vector, trigger factor, Apr, His-tag | Takara Bio |
| pK18mobSacB | Mobilizable cloning vector; pUC-oriV, mob, sacB, Kmr | [27] |
| pET23-xcv2724 | Expression vector for XeHypBA1 in pET-23b (+) | This study |
| pBIC3-xcv2728 | Expression vector for XeHypAA in pBIC3, Nmr | This study |
| pCold-xcv2729 | Expression vector for XeHypBA2 in pCold TF, Apr | This study |
| pK18-hrpX | Deletion vector for hrpX in pK18mobSacB, Kmr | This study |
| pK18-xcv2724 | Deletion vector for xehypBA1 in pK18mobSacB, Kmr | This study |
| pK18-xcv2728 | Deletion vector for xehypAA in pK18mobSacB, Kmr | This study |
| pK18-xcv2729 | Deletion vector for xehypBA2 in pK18mobSacB, Kmr | This study |
| pK18-xcv2728-2729 | Deletion vector for xehypAA and xehypAA in pK18mobSacB, Kmr | This study |
| pK18-xcv2724-2728-2729 | Deletion vector for xehypBA1, xehypAA and xehypBA2 in pK18mobSacB, Kmr | This study |
Apr, ampicillin resistance; Nmr, neomycin resistance; Kmr, kanamycin resistance