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. 2018 Jul 30;14(7):e1007563. doi: 10.1371/journal.pgen.1007563

Fig 2. Deletion of Lsm1-7/Pat1 components affects mRNA levels of the osmo-mRNAs, GPD1 and STL1, without major effects on stability.

Fig 2

A) Steady-state levels of STL1 and GPD1 mRNAs in lsm1 and pat1 mutants under osmotic stress (0.6 M KCl). mRNA levels were normalized against the housekeeping gene ACT1 and expressed relative to the highest value in wt strain. Average and standard error (SE) from three biological replicates are shown. B) Quantification of the mRNA decay rate after blocking transcription by 1,10-phenanthroline in wt and pat1 strains under osmotic stress (30 min, 0.6 M KCl) and normalized against ACT1 mRNA. Half-life ± SE from three independent experiments is indicated.