Fig 4. Deletion of PAT1 dysregulates synthesis of highly induced proteins under osmotic stress.

The accumulation of specific GFP-tagged proteins was monitored in pat1 (red line) mutants and wt (blue line) in control conditions (without stress) and under osmotic stress by addition of KCl (mild, 0.3 M; medium, 0.6 M; high, 1 M). Protein accumulation was expressed as the increment of fluorescence units relative to 5 min after addition of KCl. Green fluorescence emission (520 nm) and OD₆₀₀ was measured from the same well every 4 min for a period of 140 min in an Omega Polarstar fluorescence plate reader. To normalize the data, the OD₆₀₀ was used to remove the effect of growth differences between strains, and pat1 and wt with no GFP-tagged protein growing in parallel were used to subtract the background fluorescence. Average and SE from at least three biological replicates are shown.