Table 1.

CtDNA Analysis

Sample ID	Tissue Genotype	NGS	ddPCR-2 ml		ddPCR-200 μl
		AF (%)	AF (%)	Copies/ml	AF (%)	Copies/ml
1	p.Gly12Val	19.08	22.80	4114.00	22.10	4620.00
2	p.Gly12Ala	37.93	40.40	15708.00	39.80	14300.00
3	p.Gly13Asp	5.51	12.00	607.20	12.20	715.00
4	p.Gly13Asp	19.32	27.20	1848.00	26.50	1969.00
5	p.Gly12Val	1.75	2.40	180.40	1.60	132.00
6	p.Gly12Asp	14.24	26.80	5104.00	25.80	5573.33
7	p.Gly12Val	39.70	60.50	12430.00	60.60	9460.00
8	p.Gly12Ser	1.99	6.00	280.50	7.90	357.50
9	p.Gly13Asp	7.84	16.80	1848.00	15.00	1826.00
10	p.Gly12Ser	0.00	0.70	30.80	0.00	0.00
11	p.Gly12Asp	0.00	1.13	112.20	0.00	0.00
12	p.Gly13Asp	0.00	0.00	0.00	0.00	0.00
13	p.Gly13Asp	23.38	20.60	926.32	16.00	648.42
14	p.Gly12Ala	19.16	26.60	2917.89	25.60	2929.47
15	p.Gly12Val	31.11	44.10	7526.32	43.30	7410.53
16	p.Gly12Val	2.09	2.20	259.11	1.40	158.89
17	p.Gly13Aspa	7.55	NA		NA
18	p.Gly12Asp	NA	2.60	92.40	6.20	198.00
19	p.Gly12Val	NA	15.20	2002.00	13.20	1628.00
20	p.Gly13Asp	20.91	31.30	11088.00	30.40	11550.00
21	p.Gly12Asp	33.36	54.81	255200.00	54.40	242115.80
22	p.Gly12Val	32.27	36.80	35288.00	35.90	33770.00
23	p.Gly12Asp	NA	14.90	1650.00	13.10	1353.00
24	p.Gly12Val	3.56	8.30	682.00	7.80	759.00
25	p.Gly12Val	58.15	66.45	170720.00	NA
26	p.Gly12Ser	0.00	0.00	0.00	NA
	p.Gly12Asp	0.00	0.00	0.00	NA
	p.Gly12Alab	31.68	35.70	40304.00	35.60	39930.00
27	p.Gly13Asp	NA	13.10	875.60	11.10	957.00
28	p.Gly12Asp	0.00	0.00	0.00	0.00	0.00

Abbreviations: AF, allele frequency; ctDNA, circulating tumor DNA; ddPCR, droplet digital PCR; NA, not available; NGS, next-generation sequencing.

^aThis mutation was revealed as a c.38_39delGCinsAT and not the expected c.38G>A by NGS. The mutation was not investigated by ddPCR.

^bThe p.GlyAla was not detected by the tissue genotyping, but exclusively in the ctDNA analysis.