Fig. 2.

DNMT3Ab increases cell motility in vitro and metastasis in vivo. a–c Representative images (top) and relative bar graphs (bottom) depicting the migration and invasion of DNMT3Ab-transfected MKN45 and BGC-823 cells and the DNMT3Ab-knockdown MKN28 cells. The number of cells that migrated or invaded was counted in five fields. The migration and invasion rates are presented as the number of cells per field (*P < 0.05; **P < 0.01). d Metastatic nodules on the surface of the lung. e The number of nodules on the lungs of mice was quantified (n = 6 per group) 6 weeks after injection of DNMT3Ab-tranfected or control MKN45 cells into the tail vein. Data are shown as the mean ± SEM (***P < 0.001). f Serial sections of metastatic tumours and normal lung were stained with H&E. DNMT3Ab protein in the metastatic tumours and normal lung was detected by IHC