Fig. 5.

Metabolic changes induced by CD105 antagonism. a, b Cells were analyzed for mitochondrial activity 168 h following 4 Gy irradiation in the presence of IgG or TRC105. a Basal respiration, non-mitochondrial respiration, mitochondrial ATP, spare respiratory capacity, and b extracellular acidification rate were quantitated. Data are reported as mean ± S.D. of a representative experiment (n = 5) of three independent experiments. c Total cellular ATP was measured 0, 24, 72, 120, and 168 h following 4 Gy irradiation in the context of IgG, TRC105, or nicotinamide treatment. Data are reported as mean ± S.D. of three independent experiments (**p < 0.01, ***p < 0.001, ****p < 0.0001). d Cell cycle analysis was performed on 22Rv1 at 0, 4, 8, and 24 h post irradiation in the presence of IgG or TRC105 (n = 3) in three independent experiments