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. 2018 Aug 9;8:11904. doi: 10.1038/s41598-018-30383-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of m¹A₆₄₅ leads to structural changes within the LSU. (A) Representative gel showing structure probing with DMS or SHAPE analysis with NAI in the rrp8^G209R loss of methylation mutant. ³²P-labeled primer (helix25_StrPrb) complementary to nucleotides 2428 to 2448 of human 28S rRNA were used for the analysis. Bands corresponding to modified residues are marked in red for DMS, green for NAI and orange for both. (B) 2-D structure model with mapped modified residues as described in (A). (C) The residues sensitive to chemical modification are represented as spheres on a 3-D structure model of helix 25 (color scheme as in panel A). The model was made using Chimera and PDB file 4V88.