Table 1.

Interaction free energies determined from DMCs by using the respective mutations

Ifnar2/IFNα2	Y45A*	T46A†	I47A†	M48V†	K50A*	K55A*	H78A*†	E79A†	W102A*†	I105A*†	D106A*†
R12A					−0.1	0.9	−1.1*		−0.6*	0.8*	−1.5*
L15A		1.6	0.5	1.9		1.7	−0.7*|2.1†	1.3	−1.1*|1.8†	−0.3†
L26A	0.3	0.4	−0.5	0.4		0.3	−1.1*|−1.4†		1.1*	0.8*|0.6†	−2.1*|0.7†
F27A	2.7				−0.2	1.0			0.8*	−0.6*	0.1*
L30A		−0.4	−0.1	−1.3				1.0		0.5†	0.4†
D35A	0.7				3.4	1.6			0.5*		1.3*
K133A					−0.6	0.3			1.3*		−1.2*
R144A	1.3	0.6	−0.7	5.7				−0.2		1.5†	1.5*|0.6†
A145G		−0.7	−1.6	−0.9		1.0		−1.8	0.9*
M148A		−0.2	0.4	−0.6				−0.6	0.4†	0.3†	0.7†
R149A		−0.3	0.4	0.2				8.1		−0.1†	−0.4†
S152A	0.8	0.3	0.2	−0.1		−0.1	2.9*|4.8†	0.0	0.5*|−0.3†	−1.8†	−0.5*|0.3†
L153A		1.4	0.1	0.2		0.4	0.5*|−0.3†	1.3	1.0†	0.0†	−0.6*|1.0†

^*ΔΔG_int in kJ/mol, determined from changes in the dissociation rate constant (see Eqs. 1 and 2). 

^† ΔΔG_int in kJ/mol, determined from changes in K_D as guided by the law of mass action. Boldface indicates values of ΔΔG_int > 2.5 kJ.