Table 3.
Multiple sequence alignment of MTB (H37RV, MDR, and XDR) with DSM 20083 (Control and 2 µg, 15 µg, 25 µg, and 100 µg RIF treated samples).
| H37RV MT | INIRPVVAAIKEFFGTSQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDVHP | |
| MDR MTB | INIRPVVAAIKEFFGTSQLSQFMYQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDVHP | |
| XDR MTB | INIRPVVAAIKEFFGTSQLSQFMGQNNPLSGLTHKRRLSAPGPGGLSRERAGLEVRDVHP | |
| DSM 2008 | INIRPVNATIKEFFGTSQLSQFMDQNNPLAGVTNKRRLSALGPGGLSRDRASMEVRDVHP | |
| 2 µg DSM 20083 | INIRPVNATIKEFFGTSQLSQFMDQNNPLSGVTNKRHLSALGPGGLSRDRASMEVRDVHP | |
| 15 µg DSM 20083 | INIRPVNATIKEFFGTSQLSQFMDQNNPLSGVTNKRHLSALGPGGLSRDRASMEVRDVHP | |
| 25 µg DSM 20083 | INIRPVNATIKEFFGTSQLSQFMDQNNPLSGVTNKRHLSALGPGGLSRDRASMEVRDVHP | |
| 100 µg DSM 20083 | INIRPVNATIKEFFGTSQLSQFMDQNNPLVGVTNKRRLSALGPGGLSRDRASMEVRDVPP | |
| H37RV MTB | SHYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYRKVVDGVVSDEIVYLTADEED | |
| MDR MTB | SHYGRMCPIETPEGPSIGLIGSLSVYARVNPFGFIETPYRKVVDGVVSDEIVYLTADEED | |
| XDR MTB | SHYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYRKVVDGVVSDEIVYLTADEED | |
| DSM 20083 | SHFGRMCPIESPEGPNIGLIGSLATFGRINPFGFIETPYRKVVNGHVTDEVEYMTADRDA | |
| 2 µg DSM 20083 | SHFGRMCPIESPEGPNIGLIGSLATFGRVNPFGFIETPYRKVVNGHVTDEVEYMTADRDL | |
| 15 µg DSM 20083 | SHFGRMCPIESPEGPNIGLIGSLATFGRVNPFGFIETPYRKVVNGHVTDEVEYMTADRDL | |
| 25 µg DSM 20083 | SHFGRMCPI ESPEGPNIGLIGSL ATFGR V NPFGFIETPYRKVVNGHVTDEVEYMTADRD L | |
| 100 µg DSM 20083 | SHFGRMCPIESPEGPNIGLIGSLATFGRINPFGFIETPYRKVENGHVTDEVVYMTADREV | |
| H37RV MTB | RHVVAQANSPIDADGRFVEPRVLVRRKAGEVEYVPSSEVDYMDVSPRQMVSVATAMIPFL | |
| MDR MTB | RHVVAQANSPIDADGRFVEPRVLVRRKAGEVEYVPSSEVDYMDVSPRQMVSVATAMIPFL | |
| XDR MTB | RHVVAQANSPIDADGRFVEPRVLVRRKAGEVEYVPSSEVDYMDVSPRQMVSVATAMIPFL | |
| DSM 20083 | EHVIAQANQELDENGNFVKKQALARVGEEEAVDVPVSSVDYMDVSPRQMVSVGASLIPFL | |
| 2 µg DSM 20083 | DHVIAQANQELDENGNFVQKSALARVGEEEAVDVPVSSVDYMDVSPRQMVSLGASLIPFL | |
| 15 µg DSM 20083 | DHVIAQANQELDENGNFVQKSALARVGEEEAVDVPVSSVDYMDVSPRQMVSLGASLIPFL | |
| 25 µg DSM 20083 | DHVIAQANQELDENGNFVQKSALARVGEEEAVDVPVSSVDYMDVSPRQMVSLGASLIPFL | |
| 100 µg DSM 20083 | EHVIAQANQKLDENGNFVEKEALVRDAAGEAEDVPVEMVDYMDVSPRQMVSVGASLIPFL | |
| H37RV MTB | EHDDANRALMGANMQRQAVPLV | • DSM 20083 and Mtb(H37RV, MDR, XDR) amino acid residues |
| MDR MTB | EHDDANRALMGANMQRQAVPLV | |
| XDR MTB | EHDDANRALMGANMQRQAVPLV | • CLUSTER1(Bold underlined) |
| DSM 20083 | EHDEGHRALMGTNMQRQAVPLI | • CLUSTER2 (Italic underlined) |
| 2 µg DSM 20083 | EHDEGHRALMGTNMQRQAVPLI | • CLUSTER3 (underlined Black) |
| 15 µg DSM 20083 | EHDEGHRALMGTNMQRQAVPLI | • Control (aerial font) |
| 25 µg DSM 20083 | EHDEGHRALMGTNMQRQAVPLI | • Mutated (Bold underlined) |
| 100 µg DSM 20083 | EHDEGHRALMGTNMQRQAVPLI | • Other than a cluster |