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. 2018 Aug 9;9:3181. doi: 10.1038/s41467-018-05697-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Tracking parental H3.3 and H3.1 a Labeling scheme using H3.3- or H3.1-SNAP to follow parental H3.3 or H3.1 as described in Fig. 2. We used three different chase times: 0, 24 and 48 h (equivalent to tracking global, 24 h-old and 48 h-old histones). b Standard epifluorescence images of histones H3.3 or H3.1 after a chase of 0, 24 and 48 h. DAPI stains nuclei. Scale bars represent 10 μm. c The plots show the quantification of the fluorescence for H3.3 or H3.1 after different chase times. More than 100 nuclei were quantified. Error bars represent standard deviation. Dashed lines correspond to how the signal would decrease by cell division-dependent dilution only, determined using the growth rate of each cell line HeLa H3.3- and H3.1-SNAP