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. 2018 Aug 9;8:11926. doi: 10.1038/s41598-018-30158-6

Figure 2.

Figure 2

P-gp inhibition has no effect on FZ mediated cell death. (a) A549 cells were left untreated or treated with 10 uM FZ or 10 uM Verapamil for 6 h. Rh123 was then added and fluorescence images were acquired after washings with PBS as described under “Methods”. (b) The cells were treated as before and the fluorescence was measured at Ex507/Em529 using a Tecan multimode plate reader. (c) A549 and H460 cells were treated with 1 uM FZ in the absence or presence of 10 uM verapamil for 24 h. Cell proliferation was then measured by MTT assay.