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. 2018 Aug 9;8:11926. doi: 10.1038/s41598-018-30158-6

Figure 3.

Figure 3

FZ causes early elevation of cyclin B1 levels and induces mitotic arrest. a(i) & a(ii) A549 cells were synchronized by serum starvation for 48 h and then left untreated or treated with 1 uM FZ or 50 nM colchicine for the indicated time intervals. The cell extracts were then processed for western immunoblotting using cyclin B1, cdk1 and β-actin antibodies. b(i) & b(ii) A549 cells were treated with 1 uM FZ for the indicated time intervals and the extracts were then processed for western blotting using pH3 and β-actin antibodies. The bands were quantitated using ImageJ software. (Full-length uncropped blots are included in Supplementary Fig. S6).