Figure 5.

FZ mediated inhibition of cancer cells in vitro is affected by p53 status. (a) Human H460 or A549 cells were plated onto 96-well tissue culture plates. Cells were left untreated or treated with different doses of FZ for 48 h. Cell viability was measured by MTT assay. (b) H460, A549, H522 or H1299 cells were treated with 1 uM FZ for 48 h and cell viability was measured by MTT assay. (c) H1299 cells were transfected with p53 expression construct or with vector alone. After 16 h of transfection, cells were left untreated or treated with FZ for 24 h following which cell viability was measured by MTT assay. (d) H460 cells or primary lung epithelial cells derived from rat lung tissue were treated with 1 uM FZ for different time points as indicated. Cell viability was determined by MTT assay. The significance level was set at p < 0.05. (*p < 0.05)