Figure 9.

FZ treatment results in reduced tumorigenicity in vitro and in vivo. (a) Colony formation assay for H460 and A549 cells following treatment with 1 uM FZ for 48 h. (b) Soft agar assay following FZ treatment. Cell colonies were counted after staining with 5% crystal violet (c). (d) Tumors were established in nu/nu mice by subcutaneous injection of 5 × 10⁶ A549 cells. After the tumors were 2–4 mm in diameter, the mice were orally fed with FZ dissolved in olive oil (1 mg/mouse) every second day, whereas control animals received olive oil only. Tumor volumes were then calculated by measuring tumor dimensions using Vernier callipers. (e and f) Tumors were excised, photographed and weighed. (g) Tumor vascularity in vivo was quantified between control and FZ treated mice by measuring hemoglobin spectrophotometrically (A_590nm). (h) Tumor sections from control untreated and FZ treated mice were processed for TdT staining to identify apoptotic cells (i & iv), sections were immunostained using p53 specific antibody (ii & v), and immunohistochemistry was performed on sections for CD31 (iii & vi). i, ii & iii are sections from control mock treated mice and iv, v & vi are sections from FZ treated mice (*p < 0.05).