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. 2018 Aug 9;8:11929. doi: 10.1038/s41598-018-30166-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype characterization and the T-DNA insertion sites identification of the mterf6 mutant. (A) Phenotype of the wild-type (WT; Columbia-0), mterf6-5 and mterf6-6 mutants, and mterf6-5 complemented plants grown on MS medium with or without 2% sucrose for 7 or 14 days. Bars = 0.1 cm. (B) Phenotype of the WT, mterf6-5 and mterf6-5 complemented plant grown in soil after being grown in MS medium with 2% sucrose for 2 weeks. Bar = 1 cm. (C) Positions of the two T-DNA insertions in the mterf6 mutants and the genomic fragment for complementation experiment. The black boxes, gray boxes and lines indicate the exons, untranslated regions and introns, respectively. The location of the primer pair (LP and RP) used for genotyping is shown by arrows. (D) MTERF6 expression in WT, mterf6-5 and mterf6-6 mutants and in the complementation line.